LOCUS Construct_31 10513 bp DNA circular UNA 20-APR-2016 DEFINITION 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA. ACCESSION urn.local...1461191559113.330 VERSION urn.local...1461191559113.330 KEYWORDS . SOURCE Human ORGANISM Human . FEATURES Location/Qualifiers promoter 31..263 /created_by="User" /modified_by="User" /label="H1 promoter" misc_feature 52 /Original_Bases="G" /modified_by="User" /label="G->C" /note="Geneious type: Editing History Replacement" misc_feature 171..177 /created_by="User" /label="DSE" primer_bind 178..197 /created_by="User" /modified_by="User" /label="JP1863_T7_E1_seq_f" misc_feature 200..209 /created_by="User" /label="PSE" misc_feature 217..235 /Original_Bases /modified_by="User" /label="TetO" misc_feature 237..242 /created_by="User" /label="TATA" misc_feature 245..263 /Original_Bases /modified_by="User" /label="TetO" misc_feature 268 /created_by="User" /label="+1 TSS" /note="Geneious type: transcription_start" gRNA 269..287 /created_by="User" /modified_by="User" /label="20nt-2" misc_feature 288..363 /Original_Bases /modified_by="User" /label="stgRNA scaffold" modified_base 289..290 /created_by="User" /label="TT->GG" modified_base 315..316 /created_by="User" /label="AA->CC" misc_signal 364..369 /Original_Bases /modified_by="User" /label="Terminator" misc_feature 400..405 /Original_Bases /label /note="Geneious type: Editing History Insertion" promoter 406..724 /created_by="User" /modified_by="User" /label="U6 promoter" misc_feature 503..510 /created_by="User" /label="Enhancer" misc_feature 582..602 /created_by="User" /label="LacO" misc_feature 659..676 /created_by="User" /modified_by="User" /label="proximal element" misc_feature 677..696 /created_by="User" /label="LacO" misc_feature 718 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" modified_base 718 /Original_Bases="A" /modified_by="User" /label="A" misc_feature 720 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" modified_base 720 /Original_Bases="A" /modified_by="User" /label="A" misc_feature 722 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" modified_base 722 /Original_Bases="A" /modified_by="User" /label="A" misc_signal 725 /created_by="User" /label="Transcription start site" gRNA 726..744 /created_by="User" /modified_by="User" /label="20nt-3" misc_feature 745..820 /created_by="User" /modified_by="User" /label="stgRNA scaffold" modified_base 746..747 /created_by="User" /label="TT->GG" modified_base 772..773 /created_by="User" /label="AA->CC" terminator 821..826 /created_by="User" /modified_by="User" /label="Termination signal" misc_feature 838..857 /created_by="User" /label="LacO" CDS 890..1405 /gene="PGKp" /SECDrawAs="Gene" /SECStyleId=1 /label="PGKp CDS" misc_signal order(1416..1424,1416..1424) /created_by="User" /label="KOZAK" CDS 1425..2138 /gene="EBFP2" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="EBFP2 CDS" misc_signal 2139..2204 /created_by="User" /label="p2a" CDS 2205..2573 /created_by="User" /label="ZeoR" misc_feature 2606..3193 /created_by="User" /label="WPRE" misc_feature 3380..3401 /created_by="User" /modified_by="User" /label="U3PPt" misc_feature 3380..3395 /created_by="User" /label="cPPT" LTR 3717..3897 /created_by="User" /label="truncHIV-1_3_LTR" terminator 3926..4153 /created_by="User" /label="bGH_PA_terminator" rep_origin 4216..4522 /created_by="User" /label="f1_origin" misc_feature complement(4642..4857) /created_by="User" /label="SV40 enhancer" rep_origin 4821..4898 /created_by="User" /label="SV40 origin" promoter 5016..5083 /created_by="User" /label="EM7 promoter" gene 5084..5455 /created_by="User" /modified_by="User" /label="bleo" terminator 5591..5710 /created_by="User" /label="SV40_PA_terminator" promoter complement(5807..5836) /created_by="User" /modified_by="User" /label="lac_promoter" CDS complement(6919..7779) /created_by="User" /label="AmpR" promoter complement(7821..7848) /created_by="User" /label="AmpR promoter" promoter 8151..8727 /created_by="User" /modified_by="User" /label="CMV_imm_early promoter" misc_feature 8230..8517 /created_by="User" /label="CAG enhancer" LTR 8747..8927 /created_by="User" /modified_by="User" /label="HIV-1_5_LTR" misc_feature 9038..9082 /created_by="User" /modified_by="User" /label="HIV-1_psi_pack" misc_feature 9592..9825 /created_by="User" /label="RRE" misc_feature 10356..10371 /created_by="User" /label="cPPT" ORIGIN 1 gcgaacgctc ctcaccatac gaaagacctt gaatcctatg cttcgaacgc tcacgtcatc 61 aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc 121 gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt 181 cgctatgtgt tctgggaaat caccataaac gtgaaatccc tatcagtgat agagacttat 241 aagttcccta tcagtgatag agatcccagt ggctttacca acagtacggg ttagagctag 301 aaatagcaag ttaacctaag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg 361 tgcttttttt cacgaggcgg acactgattg acacggtttg ctagctgtac aaaaaagcag 421 gctttaaagg aaccaattca gtcgactgga tccggtacca aggtcgggca ggaagagggc 481 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt 541 agaattaatt tgactgtaaa cacaaagata ttagtacaaa aaattgtgag cggataacaa 601 ttatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt 661 accgtaactt gaaagtaatt gtgagcgctc acaattatat atcttgtgga aaggacgaaa 721 caccgagtcg cgtgtagcga agcagggtta gagctagaaa tagcaagtta acctaaggct 781 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttctag acccagcaat 841 tgtgagcgct cacaatttac aaagttggca ctcactcgct attcgaacta attctaccgg 901 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 961 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 1021 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 1081 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 1141 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 1201 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 1261 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 1321 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 1381 tcatctccgg gcctttcgac ctgcatccca agcttgccac catggtgagc aagggcgagg 1441 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca 1501 agttcagcgt gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt 1561 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc 1621 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt 1681 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct 1741 acaagacccg cgccgaggtg aagttcgagg gcgacaccct agtgaaccgc atcgagctga 1801 agggcgtcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca 1861 acagccacaa catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca 1921 agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca 1981 cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg 2041 tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg 2101 ccgccgggat cactctcggc atggacgagc tgtacaaggg ctctggtgca accaatttct 2161 ctcttcttaa acaagccggt gatgtggagg agaaccccgg acccgccaag ttgaccagtg 2221 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc 2281 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga 2341 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt 2401 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc 2461 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg 2521 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgataag 2581 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 2641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 2701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 2761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 2821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 2881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 2941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 3001 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 3061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3121 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3181 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa 3241 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg 3301 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg 3361 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 3421 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc 3481 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc 3541 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt 3601 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt 3661 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt 3721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 3781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 3841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg 3901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 3961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 4021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 4081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 4141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 4561 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 4981 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 5041 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag 5101 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg 5161 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt 5221 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 5281 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 5341 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 5401 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 5461 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 5521 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 5581 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 5641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5701 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 5761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 5821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 5881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 5941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 6361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg 7921 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca 7981 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc 8041 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg 8101 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 8161 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 8221 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 8281 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 8341 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 8401 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 8461 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 8521 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 8581 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 8641 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 8701 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt 8761 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 8821 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 8881 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac 8941 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct 9001 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact 9061 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt 9121 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa 9181 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa 9241 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca 9301 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata 9361 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag 9421 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa 9481 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 9541 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 9601 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 9661 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 9721 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 9781 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 9841 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc 9901 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta 9961 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca 10021 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg 10081 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt 10141 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca 10201 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg 10261 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat 10321 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt 10381 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac 10441 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag 10501 tttggttaat taa //