LOCUS Construct_3 10776 bp DNA circular UNA 20-APR-2016 DEFINITION U6p_20nt1_mod2_sgRNA (stgRNA). ACCESSION urn.local...1461188663766.11 VERSION urn.local...1461188663766.11 KEYWORDS . SOURCE Human ORGANISM Human . FEATURES Location/Qualifiers promoter 239..815 /created_by="User" /modified_by="User" /label="CMV_imm_early promoter" misc_feature 318..605 /created_by="User" /label="CAG enhancer" LTR 835..1015 /created_by="User" /modified_by="User" /label="HIV-1_5_LTR" misc_feature 1126..1170 /created_by="User" /modified_by="User" /label="HIV-1_psi_pack" misc_feature 1680..1913 /created_by="User" /label="RRE" misc_feature 2444..2459 /created_by="User" /label="cPPT" primer_bind 2571..2590 /Mismatches=0 /created_by="primer3" /annotation_group="141566513778868200028694d1d-6973-402e-b 821-c28ce9b7852b" /%GC="55.0" /Tm="59.1" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Sequence="GGGACAGCAGAGATCCAGTT" /label="JP1706" primer_bind 2577..2607 /Mismatches=0 /created_by="primer3" /annotation_group="14158103166042940003272ff1b-6c61-4f45-a fa8-195ac9b99404" /%GC="64.5" /Tm="75.2" /Hairpin_Tm="47.7" /Self_Dimer_Tm="8.8" /Sequence="GCAGAGATCCAGTTTGGGGGGTTCCGCGCAC" /label="JP1710" primer_bind 2594..2616 /created_by="primer3" /%GC="69.6" /Tm="64.72" /Hairpin="4.0" /Primer-Dimer="4.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Sequence="GGGGTTCCGCGCACATTTCCCCG" /Mismatches=0 /annotation_group="PrimerPlugin Group3c68cbfa-121b-4872-a7ab-08a32877e25e_urn:local:.:1365 712513487.384" /label="JP313" CDS complement(<2594..2598) /gene="AMP" /SECDrawAs="Gene" /SECStyleId=1 /label="AMP CDS" promoter 2630..2940 /created_by="User" /modified_by="User" /label="U6 promoter" misc_feature 2727..2734 /created_by="User" /label="Enhancer" misc_feature 2883..2901 /created_by="User" /label="proximal element" misc_feature 2919..2924 /created_by="User" /label="TATA box" gRNA 2949..2967 /created_by="User" /modified_by="User" /label="20nt1" misc_feature 2968..3044 /created_by="User" /modified_by="User" /label="stgRNA scaffold" modified_base 2969..2970 /created_by="User" /modified_by="User" /label="TT->GG" modified_base 2995..2996 /created_by="User" /label="AA->CC" terminator 3045..3049 /created_by="User" /label="Termination signal" misc_feature complement(3074..3105) /Mismatches=0 /created_by="primer3" /annotation_group="141581031682964600096881a94-d9ce-450e-9 300-407e04f37bb8" /%GC="53.1" /Tm="69.7" /Hairpin_Tm="30.8" /Self_Dimer_Tm="11.5" /Sequence="CCCGGTAGAATTCCTCGACGTCTAATGCCAAC" /label="JP1711" /note="Geneious type: primer_bind_reverse" misc_feature complement(3087..3106) /Mismatches=0 /created_by="primer3" /annotation_group="1415665137960342000e062635e-3225-4b00-a 339-35365797f3ae" /%GC="55.0" /Tm="58.9" /Hairpin_Tm="None" /Self_Dimer_Tm="12.3" /Sequence="ACCCGGTAGAATTCCTCGAC" /label="JP1707" /note="Geneious type: primer_bind_reverse" CDS 3094..3609 /gene="PGKp" /SECDrawAs="Gene" /SECStyleId=1 /label="PGKp CDS" misc_feature complement(3169..3193) /Hairpin="6.0" /Primer-Dimer="2.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Mismatches=0 /created_by="primer3" /annotation_group="14158097666895440008bddd82b-4d20-4bb2-8 efa-9fa8e0328024" /%GC="68.0" /Tm="71.6" /Hairpin_Tm="64.2" /Self_Dimer_Tm="21.7" /Sequence="GGCCAGAGGCCACTTGTGTAGCGCC" /label="JP467" /note="Geneious type: primer_bind_reverse" misc_signal order(3620..3628,3620..3628) /created_by="User" /label="KOZAK" CDS 3629..4342 /gene="EBFP2" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="EBFP2 CDS" sig_peptide 4343..4408 /created_by="User" /modified_by="User" /label="p2a" CDS 4409..5440 /created_by="User" /label="hygroR" misc_feature 5470..6057 /created_by="User" /label="WPRE" misc_feature 6244..6265 /created_by="User" /modified_by="User" /label="U3PPt" misc_feature 6244..6259 /created_by="User" /label="cPPT" LTR 6581..6761 /created_by="User" /label="truncHIV-1_3_LTR" terminator 6790..7017 /created_by="User" /label="bGH_PA_terminator" rep_origin 7080..7386 /created_by="User" /label="f1_origin" misc_feature complement(7506..7721) /created_by="User" /label="SV40 enhancer" rep_origin 7685..7762 /created_by="User" /label="SV40 origin" promoter 7880..7947 /created_by="User" /label="EM7 promoter" gene 7948..8319 /created_by="User" /modified_by="User" /label="bleo" terminator 8455..8574 /created_by="User" /label="SV40_PA_terminator" promoter complement(8671..8700) /created_by="User" /modified_by="User" /label="lac_promoter" CDS complement(9783..10643) /created_by="User" /label="AmpR" promoter complement(10685..10712) /created_by="User" /label="AmpR promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggggggttc cgcgcacatt tccccgaaaa gtgccacctt gtacaaaaaa 2641 gcaggcttta aaggaaccaa ttcagtcgac tggatccggt accaaggtcg ggcaggaaga 2701 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat 2761 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa 2821 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat 2881 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg 2941 aaacaccgta agtcggagta ctgtcctggg ttagagctag aaatagcaag ttaacctaag 3001 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttc tagacccagc 3061 tttcttgtac aaagttggca ttagacgtcg aggaattcta ccgggtaggg gaggcgcttt 3121 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa 3181 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct 3241 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc 3301 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc 3361 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc 3421 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc 3481 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct 3541 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt 3601 cgacctgcat cccaagcttg ccaccatggt gagcaagggc gaggagctgt tcaccggggt 3661 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgagggg 3721 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg 3781 caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg tgcagtgctt 3841 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 3901 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga cccgcgccga 3961 ggtgaagttc gagggcgaca ccctagtgaa ccgcatcgag ctgaagggcg tcgacttcaa 4021 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacatcta 4081 tatcatggcc gtcaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacgt 4141 ggaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 4201 ccccgtgctg ctgcccgaca gccactacct gagcacccag tccgtgctga gcaaagaccc 4261 caacgagaag cgcgatcaca tggtcctgct ggagttccgc accgccgccg ggatcactct 4321 cggcatggac gagctgtaca agggaagcgg agctactaac ttcagcctgc tgaagcaggc 4381 tggagacgtg gaggagaacc ctggacctaa aaagcctgaa ctcaccgcga cgtctgtcga 4441 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga 4501 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag 4561 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct 4621 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc 4681 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct 4741 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg 4801 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg 4861 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc 4921 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg 4981 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac 5041 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat 5101 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag 5161 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga 5221 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg 5281 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag 5341 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg 5401 ccccagcact cgtccggatc gggagatggg ggaggctaac taagaattcg atatcaagct 5461 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5521 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5581 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5641 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5701 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5761 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5821 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 5881 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5941 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6001 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg 6061 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 6121 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 6181 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6241 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct 6301 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg 6361 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca 6421 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct 6481 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc 6541 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca 6601 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6661 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6721 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct 6781 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 6841 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 6901 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 6961 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 7021 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 7081 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 7141 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 7201 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 7261 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 7321 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 7381 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 7441 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 7501 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 7561 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 7681 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 7741 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 7801 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 7861 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta 7921 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc 7981 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg 8041 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc 8101 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg 8161 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg 8221 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg 8281 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc 8341 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 8401 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 8461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 8521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 8581 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 8641 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 8701 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 8761 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 8821 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 8881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 8941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 9001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 9061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 9121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 9181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 9241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 9301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 9361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 9421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 9481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 9541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 9601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 9661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 9721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 9781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 9841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 9901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 9961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 10021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 10081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 10141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 10201 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 10261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 10321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 10381 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 10441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 10501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 10561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 10621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 10681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 10741 gttccgcgca catttccccg aaaagtgcca cctgac //