LOCUS Construct_28 11427 bp DNA circular UNA 20-APR-2016 DEFINITION hUBCp_TetR_p2a_LacI_p2a_ZeoR. ACCESSION urn.local...1461191418033.310 VERSION urn.local...1461191418033.310 KEYWORDS . SOURCE Human ORGANISM Human . FEATURES Location/Qualifiers promoter 239..815 /created_by="User" /modified_by="User" /label="CMV_imm_early promoter" misc_feature 318..605 /created_by="User" /label="CAG enhancer" LTR 835..1015 /created_by="User" /modified_by="User" /label="HIV-1_5_LTR" misc_feature 1126..1170 /created_by="User" /modified_by="User" /label="HIV-1_psi_pack" ORF 1558..2445 /created_by="User" /label="ORF frame 1" misc_feature 1680..1913 /created_by="User" /label="RRE" misc_feature 2444..2459 /created_by="User" /label="cPPT" promoter 2618..3821 /created_by="User" /modified_by="User" /label="hUBC promoter" misc_signal 3842..3847 /created_by="User" /label="KOZAK" gene 3848..4468 /created_by="User" /label="TetR" misc_feature 4469..4474 /created_by="User" /label="BsiWI" misc_signal 4475..4540 /created_by="User" /label="p2a" gene 4541..5617 /created_by="User" /label="LacI" primer_bind 5290..5321 /created_by="User" /label="JP1889" sig_peptide 5633..5653 /created_by="User" /modified_by="User" /label="NLS" misc_signal 5654..5719 /created_by="User" /label="p2a" CDS 5720..6088 /created_by="User" /label="ZeoR" misc_feature 6121..6708 /created_by="User" /label="WPRE" misc_feature 6895..6916 /created_by="User" /modified_by="User" /label="U3PPt" misc_feature 6895..6910 /created_by="User" /label="cPPT" LTR 7232..7412 /created_by="User" /label="truncHIV-1_3_LTR" terminator 7441..7668 /created_by="User" /label="bGH_PA_terminator" rep_origin 7731..8037 /created_by="User" /label="f1_origin" misc_feature complement(8157..8372) /created_by="User" /label="SV40 enhancer" rep_origin 8336..8413 /created_by="User" /label="SV40 origin" promoter 8531..8598 /created_by="User" /label="EM7 promoter" gene 8599..8970 /created_by="User" /modified_by="User" /label="bleo" terminator 9106..9225 /created_by="User" /label="SV40_PA_terminator" promoter complement(9322..9351) /created_by="User" /modified_by="User" /label="lac_promoter" CDS complement(10434..11294) /created_by="User" /label="AmpR" promoter complement(11336..11363) /created_by="User" /label="AmpR promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg 2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc 2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga 3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg 3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga 3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc 3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt 3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg 3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg 3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg 3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga 3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg 3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat 3781 tgtccgctaa attctggccg tttttggctt ttttgttaga caggatcccc gggtaccggt 3841 cgccaccatg tctcggttgg acaaatctaa agtaatcaac tctgcactgg aattgctgaa 3901 cgaggtaggc atagagggcc tcacaacgag gaagctggcc caaaagctgg gcgtcgaaca 3961 gccaaccctg tactggcacg tcaagaataa aagggctctc ctggacgcgc tggcaattga 4021 gatgctcgac agacaccata cacacttttg cccccttgaa ggggaatcct ggcaggactt 4081 cctgcgaaac aatgccaagt catttagatg cgctcttctg tctcatcggg acggtgctaa 4141 ggtgcatctg ggtacaagac ccacggaaaa gcagtatgag acactggaaa atcaactggc 4201 ctttttgtgt cagcagggct tctctctcga aaacgcgctt tacgcgctgt cagccgtggg 4261 tcattttacc ctgggctgcg tgctggagga ccaggagcat caagtggcta aggaggaacg 4321 ggaaacccct accaccgact ctatgccacc tctcttgcgg caggcaattg agttgttcga 4381 ccaccagggt gccgagccgg ccttcctgtt cggcttggag cttatcatct gcggcctgga 4441 gaagcagctg aagtgtgaga gtggaagtcg tacgggaagc ggagctacta acttcagcct 4501 gctgaagcag gctggagacg tggaggagaa ccctggacct aaaccagtaa cattgtatga 4561 tgtcgcagag tatgccggtg tctcttatca gactgtttcc agagtggtga accaggccag 4621 ccatgtttct gccaaaacca gggaaaaagt ggaagcagcc atggcagagc tgaattacat 4681 tcccaacaga gtggcacaac aactggcagg caaacagagc ttgctgattg gagttgccac 4741 ctccagtctg gccctgcatg caccatctca aattgtggca gccattaaat ctagagctga 4801 tcaactggga gcctctgtgg tggtgtcaat ggtagaaaga agtggagttg aagcctgtaa 4861 agctgctgtg cacaatcttc tggcacaaag agtcagtggg ctgatcatta actatccact 4921 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccag cactctttct 4981 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag atggtacaag 5041 actgggtgtg gagcatctgg ttgcattggg acaccagcaa attgcactgc ttgcgggccc 5101 actcagttct gtctcagcaa ggctgagact ggctggctgg cataaatatc tcactaggaa 5161 tcaaattcag ccaatagctg aaagagaagg ggactggagt gccatgtctg ggtttcaaca 5221 aaccatgcaa atgctgaatg agggcattgt tcccactgca atgctggttg ccaatgatca 5281 gatggcactg ggtgcaatga gagccattac tgagtctggg ctgagagttg gtgcagatat 5341 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac 5401 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc 5461 tcagggccag gcggtgaagg gcaatcagct gttgccagtc tcactggtga agagaaaaac 5521 caccctggca cccaatacac aaactgcctc tccccgggca ttggctgatt cactcatgca 5581 gctagcaaga caggtttcca gactggaaag tgggcagagc agcctgaggc ctcctaagaa 5641 gaagaggaag gttggctctg gtgcaaccaa tttctctctt cttaaacaag ccggtgatgt 5701 ggaggagaac cccggacccg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga 5761 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga 5821 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga 5881 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta 5941 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac 6001 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg 6061 cgtgcacttc gtggccgagg agcaggactg ataagaattc gatatcaagc ttatcgataa 6121 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 6181 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 6241 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 6301 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 6361 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 6421 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 6481 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 6541 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 6601 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 6661 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg 6721 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 6781 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca 6841 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 6901 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg 6961 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat 7021 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt 7081 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat 7141 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca 7201 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc 7261 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 7321 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 7381 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct 7441 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 7501 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 7561 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 7621 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 7681 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 7741 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 7801 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 7861 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 7921 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 7981 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 8041 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 8101 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 8161 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 8221 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 8281 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 8341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 8401 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 8461 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 8521 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac 8581 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc 8641 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg 8701 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag 8761 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg 8821 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg 8881 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac 8941 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc 9001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 9061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 9241 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 9301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 9361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 9421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 9481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 9541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 9601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 9661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 9721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 9781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 9841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 9901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 9961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 10021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 10081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 10141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 10201 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 10261 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 10321 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 10381 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 10441 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 10501 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 10561 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 10621 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 10681 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 10741 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 10801 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 10861 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 10921 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 10981 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 11041 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 11101 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 11161 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 11221 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 11281 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 11341 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 11401 acatttcccc gaaaagtgcc acctgac //