LOCUS Construct_25 11090 bp DNA circular UNA 20-APR-2016 DEFINITION U6p_20nt_sgRNA_target. ACCESSION urn.local...1461190572731.210 VERSION urn.local...1461190572731.210 KEYWORDS . SOURCE Human ORGANISM Human . FEATURES Location/Qualifiers promoter 239..815 /created_by="User" /modified_by="User" /label="CMV_imm_early promoter" misc_feature 318..605 /created_by="User" /label="CAG enhancer" LTR 835..1015 /created_by="User" /modified_by="User" /label="HIV-1_5_LTR" misc_feature 1126..1170 /created_by="User" /modified_by="User" /label="HIV-1_psi_pack" misc_feature 1680..1913 /created_by="User" /label="RRE" misc_feature 2444..2459 /created_by="User" /label="cPPT" primer_bind 2571..2590 /Mismatches=0 /created_by="primer3" /annotation_group="141566513778868200028694d1d-6973-402e-b 821-c28ce9b7852b" /%GC="55.0" /Tm="59.1" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Sequence="GGGACAGCAGAGATCCAGTT" /label="JP1706" primer_bind 2577..2607 /Mismatches=0 /created_by="primer3" /annotation_group="14158103166042940003272ff1b-6c61-4f45-a fa8-195ac9b99404" /%GC="64.5" /Tm="75.2" /Hairpin_Tm="47.7" /Self_Dimer_Tm="8.8" /Sequence="GCAGAGATCCAGTTTGGGGGGTTCCGCGCAC" /label="JP1710" primer_bind 2594..2616 /created_by="primer3" /%GC="69.6" /Tm="64.72" /Hairpin="4.0" /Primer-Dimer="4.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Sequence="GGGGTTCCGCGCACATTTCCCCG" /Mismatches=0 /annotation_group="PrimerPlugin Group3c68cbfa-121b-4872-a7ab-08a32877e25e_urn:local:.:1365 712513487.384" /label="JP313" CDS complement(<2594..2598) /gene="AMP" /SECDrawAs="Gene" /SECStyleId=1 /label="AMP CDS" promoter 2630..2940 /created_by="User" /modified_by="User" /label="U6 promoter" misc_feature 2727..2734 /created_by="User" /label="Enhancer" misc_feature 2883..2901 /created_by="User" /label="proximal element" misc_feature 2919..2924 /created_by="User" /label="TATA box" gRNA 2948..2967 /created_by="User" /modified_by="User" /label="20nt-1" misc_feature 2968..3044 /created_by="User" /modified_by="User" /label="sgRNA scaffold" terminator 3045..3050 /created_by="User" /label="Termination signal" gRNA 3263..3282 /created_by="User" /label="20bp" misc_signal 3283..3285 /created_by="User" /label="PAM" CDS 3408..3923 /gene="PGKp" /SECDrawAs="Gene" /SECStyleId=1 /label="PGKp CDS" misc_feature complement(3483..3507) /Hairpin="6.0" /Primer-Dimer="2.0" /Monovalent_Salt_Concentration="50.0" /Divalent_Salt_Concentration="0.0" /DNTP_Concentration="0.0" /Mismatches=0 /created_by="primer3" /annotation_group="14158097666895440008bddd82b-4d20-4bb2-8 efa-9fa8e0328024" /%GC="68.0" /Tm="71.6" /Hairpin_Tm="64.2" /Self_Dimer_Tm="21.7" /Sequence="GGCCAGAGGCCACTTGTGTAGCGCC" /label="JP467" /note="Geneious type: primer_bind_reverse" misc_signal order(3934..3942,3934..3942) /created_by="User" /label="KOZAK" CDS 3943..4656 /gene="EBFP2" /SECDrawAs="Gene" /SECStyleId=1 /modified_by="User" /label="EBFP2 CDS" sig_peptide 4657..4722 /created_by="User" /modified_by="User" /label="p2a" CDS 4723..5754 /created_by="User" /label="hygroR" misc_feature 5784..6371 /created_by="User" /label="WPRE" misc_feature 6558..6579 /created_by="User" /modified_by="User" /label="U3PPt" misc_feature 6558..6573 /created_by="User" /label="cPPT" LTR 6895..7075 /created_by="User" /label="truncHIV-1_3_LTR" terminator 7104..7331 /created_by="User" /label="bGH_PA_terminator" rep_origin 7394..7700 /created_by="User" /label="f1_origin" misc_feature complement(7820..8035) /created_by="User" /label="SV40 enhancer" rep_origin 7999..8076 /created_by="User" /label="SV40 origin" promoter 8194..8261 /created_by="User" /label="EM7 promoter" gene 8262..8633 /created_by="User" /modified_by="User" /label="bleo" terminator 8769..8888 /created_by="User" /label="SV40_PA_terminator" promoter complement(8985..9014) /created_by="User" /modified_by="User" /label="lac_promoter" CDS complement(10097..10957) /created_by="User" /label="AmpR" promoter complement(10999..11026) /created_by="User" /label="AmpR promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggggggttc cgcgcacatt tccccgaaaa gtgccacctt gtacaaaaaa 2641 gcaggcttta aaggaaccaa ttcagtcgac tggatccggt accaaggtcg ggcaggaaga 2701 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat 2761 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa 2821 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat 2881 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg 2941 aaacaccgta agtcggagta ctgtcctgtt ttagagctag aaatagcaag ttaaaataag 3001 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ctagaatcgc 3061 taaactgcgt cgcggagcct tatggcatag gtcgtccgcg gagcattccg gtaacgctta 3121 tggtccatag cacattcatc gcatccgggc gtgcgctcta tttgacgatc ccttggcgca 3181 gaggtgctgg ccacgtgcta aattaaagcg gctgcactac tgtaaggtcc gtcggccgtc 3241 gatccaccga ttcgcgtcgt gcgtaagtcg gagtactgtc ctggggctag catgtggaca 3301 agccagcata gccatttaac tcactcgcta ttcgaactca acgctacggt cgccgagtgg 3361 tacgcgtacc cagctttctt gtacaaagtt ggcattagac gtcgaggaat tctaccgggt 3421 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 3481 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 3541 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 3601 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 3661 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 3721 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 3781 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 3841 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 3901 atctccgggc ctttcgacct gcatcccaag cttgccacca tggtgagcaa gggcgaggag 3961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 4021 ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc 4081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac 4141 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 4201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac 4261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat cgagctgaag 4321 ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac 4381 agccacaaca tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag 4441 atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc 4501 cccatcggcg acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg 4561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc 4621 gccgggatca ctctcggcat ggacgagctg tacaagggaa gcggagctac taacttcagc 4681 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctaaaaagcc tgaactcacc 4741 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag 4801 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc 4861 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt 4921 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg 4981 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa 5041 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt 5101 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg 5161 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac 5221 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac 5281 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac 5341 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac 5401 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc 5461 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc 5521 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct 5581 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca 5641 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat 5701 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactaagaa 5761 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact 5821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 6001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 6061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 6121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 6181 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 6241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 6301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 6361 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt 6421 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 6481 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 6541 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 6601 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag 6661 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta 6721 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta 6781 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt 6841 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct 6901 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6961 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7021 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc 7081 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 7141 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 7201 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 7261 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 7321 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 7381 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 7441 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 7501 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 7561 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 7621 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 7681 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 7741 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 7801 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 7861 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 7921 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 7981 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 8041 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 8101 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 8161 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt 8221 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg 8281 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc 8341 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga 8401 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt 8461 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc 8521 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg 8581 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg 8641 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 8701 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 8761 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8881 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 8941 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 9001 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 9061 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 9121 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 9181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 9241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 9301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 9361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 9421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 9481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 9541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 9601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 9661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 9721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 9781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 9841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 9901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 9961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 10021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 10081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 10141 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 10201 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 10261 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 10321 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 10381 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 10441 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 10501 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 10561 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 10621 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 10681 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 10741 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 10801 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 10861 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 10921 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 10981 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 11041 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac //