LOCUS Construct_12 14118 bp DNA circular UNA 20-APR-2016 DEFINITION hUBCp-Cas9-3xNLS_p2a_puroR. ACCESSION urn.local...1417926308651.146 (modified) VERSION urn.local...1417926308651.146 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers misc_feature 520..535 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="cPPT" CDS 520..535 /gene="cPPT" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="cPPT CDS" primer_bind 625..658 /Mismatches=0 /created_by="primer3" /annotation_group="141893905641164100034dc49e3-d075-4f8c-8 3a4-b34d84d9e890" /%GC="41.2" /Tm="66.4" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Sequence="CAAAATTTTCGGGTTTATTACAGGGACAGCAGAG" /label="JP1208" promoter 694..1899 /created_by="User" /modified_by="User" /label="hUBC promoter" misc_signal 1957..1962 /created_by="User" /modified_by="User" /label="Kozak" primer_bind 1957..1984 /Mismatches=0 /Extension="TGTACGGTGGGAGGCCTATATAAGCAACCGGT" /created_by="primer3" /annotation_group="1418939221963244000ad2d339e-145a-4c25-8 d02-0359e0331ed5" /%GC="53.6" /Tm="67.6" /Hairpin_Tm="46.4" /Self_Dimer_Tm="4.1" /Sequence="GCCACCATGGACAAGAAGTACAGCATCG" /label="JP1744" CDS 1963..6066 /note="hSpCas9" /modified_by="User" /Transferred_From="SPM1388" /Transferred_Similarity="100.00%" /label="hSpCas9" primer_bind 2526..2553 /Mismatches=0 /created_by="primer3" /annotation_group="1418939056609506000074a3087-e211-4ce1-9 4af-950c1281af58" /%GC="57.1" /Tm="68.8" /Hairpin_Tm="39.5" /Self_Dimer_Tm="3.5" /Sequence="GGTGCAGACCTACAACCAGCTGTTCGAG" /label="JP1663" primer_bind 3192..3222 /Mismatches=0 /created_by="primer3" /annotation_group="1418939056791816000661226d9-ec05-433a-9 445-78d6df11fc61" /%GC="67.7" /Tm="75.5" /Hairpin_Tm="53.8" /Self_Dimer_Tm="21.3" /Sequence="CCCCCACCAGATCCACCTGGGAGAGCTGCAC" /label="JP1664" primer_bind 3798..3831 /Mismatches=0 /created_by="primer3" /annotation_group="1418939056968037000e7e4578b-e9ef-46eb-b 3f6-e76bde882536" /%GC="52.9" /Tm="70.5" /Hairpin_Tm="None" /Self_Dimer_Tm="14.4" /Sequence="CGAGGACATTCTGGAAGATATCGTGCTGACCCTG" /label="JP1665" primer_bind 4428..4461 /Mismatches=0 /modified_by="User" /created_by="primer3" /annotation_group="1418939057138654000b118c7cc-14fd-4c11-8 1b0-461d774dd16d" /%GC="58.8" /Tm="73.7" /Hairpin_Tm="78.4" /Self_Dimer_Tm="13.9" /Sequence="GTACGTGGACCAGGAACTGGACATCAACCGGCTG" /label="JP1666" primer_bind 5105..5136 /Mismatches=0 /modified_by="User" /created_by="primer3" /annotation_group="141893905732118300046750ae8-2253-4daa-a 9cc-485e302716f1" /%GC="65.6" /Tm="75.4" /Hairpin_Tm="53.7" /Self_Dimer_Tm="19.1" /Sequence="CCGAGATTACCCTGGCCAACGGCGAGATCCGG" /label="JP1667" primer_bind 5805..5838 /Mismatches=0 /modified_by="User" /created_by="primer3" /annotation_group="1418939057490327000a13f334c-6525-4134-a 462-23343bfcf1c9" /%GC="58.8" /Tm="74.1" /Hairpin_Tm="48.4" /Self_Dimer_Tm="15.0" /Sequence="CCTGGCCGACGCTAATCTGGACAAAGTGCTGTCC" /label="JP1668" sig_peptide 6067..6114 /created_by="User" /modified_by="User" /Transferred_From="SPM1388" /Transferred_Similarity="100.00%" /label="NLS" misc_feature 6115..6138 /created_by="User" /Transferred_From="SPM1388" /Transferred_Similarity="100.00%" /label="linker" sig_peptide 6139..6159 /created_by="User" /Transferred_From="SPM1388" /Transferred_Similarity="100.00%" /label="NLS" sig_peptide 6166..6186 /created_by="User" /Transferred_From="SPM1388" /Transferred_Similarity="100.00%" /label="NLS" sig_peptide 6196..6261 /created_by="User" /label="p2a" CDS 6262..6855 /created_by="User" /Transferred_From="SPM1388" /Transferred_Similarity="100.00%" /label="puroR" misc_feature 6888..7475 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="WPRE" misc_feature complement(6953..6980) /Mismatches=0 /created_by="primer3" /annotation_group="1418939057665054000811d4f3b-82f3-45f6-b 6e8-60760aef28ca" /%GC="50.0" /Tm="66.4" /Hairpin_Tm="35.8" /Self_Dimer_Tm="4.9" /Sequence="GGCATTAAAGCAGCGTATCCACATAGCG" /label="JP702" /note="Geneious type: primer_bind_reverse" misc_feature 7662..7683 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="U3PPt" misc_feature 7662..7677 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="cPPT" CDS 7662..7683 /gene="U3PPT" /product="reverse transcriptase-associated RNase H cleavage of the RNA at the polypurine tract (PPT), a short (ca. 11 to 19 nucleotides [nt]) string of purines located at the upstream border of the 3′ long terminal repeat" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="U3PPT CDS" CDS 7662..7677 /gene="cPPT" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="cPPT CDS" LTR 7999..8179 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /modified_by="User" /label="HIV-1_3_LTR" CDS 7999..8179 /gene="5'LTR; 5'LTR; del3'LTR; del3'LTR" /product="HIV-1_5_LTR; HIV-1_5_LTR; truncHIV-1_3_LTR; truncHIV-1_3_LTR" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="5'LTR CDS; 5'LTR CDS; del3'LTR CDS; del3'LTR CDS" terminator 8208..8435 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="bGH_PA_terminator" CDS 8208..8435 /gene="bGH-Ter" /product="bGH_PA_terminator" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="bGH-Ter CDS" rep_origin 8498..8804 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="f1_origin" misc_signal complement(8924..9139) /SECDrawAs="Label" /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="misc signal" misc_feature complement(8924..9139) /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="SV40 enhancer" CDS 8936..9204 /gene="SV40p" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="SV40p CDS" rep_origin 9103..9180 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="SV40 origin" misc_feature 9103..9180 /gene="SV40-Ori" /SECDrawAs="Region" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="SV40-Ori misc" promoter 9298..9365 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="EM7 promoter" CDS 9298..9365 /gene="EM7p" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="EM7p CDS" gene 9366..9737 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="bleo" CDS 9366..9737 /gene="Bleo" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="Bleo CDS" gene 9369..9737 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335" /X-Transferred_Similarity="100.00%" /created_by="User" /Transferred_From="M1399, SPM1335" /Transferred_Similarity="100.00%" /label="Ble Sh (Zeo Resistance); Zeo" terminator 9873..9992 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="SV40_PA_terminator" CDS 9873..9992 /gene="SV40-pA" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="SV40-pA CDS" promoter complement(10089..10118) /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="lac_promoter" gene complement(11201..12061) /created_by="User" /X-Transferred_From="SPM1381" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1381" /Transferred_Similarity="100.00%" /label="AmpR" CDS complement(11201..12061) /gene="Amp" /SECDrawAs="Gene" /SECStyleId=1 /created_by="User" /X-Transferred_From="Plasmid_p119 (modified); SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified), SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="Amp CDS; Amp CDS; AmpR; AmpR" promoter complement(12103..12130) /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="AmpR promoter" CDS complement(12103..12131) /gene="Ampp" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="Ampp CDS" promoter 12433..13009 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="CMV_imm_early promoter" CDS 12433..13009 /gene="CMVp" /product="CMV_immearly_promoter" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="CMVp CDS" misc_signal 12512..12799 /SECDrawAs="Label" /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="misc signal" misc_feature 12512..12799 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="CAG enhancer" LTR 13029..13209 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /modified_by="User" /label="HIV-1_5_LTR" CDS 13029..13209 /gene="5'LTR; 5'LTR; del3'LTR; del3'LTR" /product="HIV-1_5_LTR; HIV-1_5_LTR; truncHIV-1_3_LTR; truncHIV-1_3_LTR" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="5'LTR CDS; 5'LTR CDS; del3'LTR CDS; del3'LTR CDS" misc_feature 13320..13364 /created_by="User" /modified_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="HIV-1_psi_pack" CDS 13320..13364 /gene="Psi" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="Psi CDS" misc_feature 13874..14107 /created_by="User" /X-Transferred_From="SPM1335; SPM1388" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, SPM1335, SPM1388" /Transferred_Similarity="100.00%" /label="RRE" CDS 13874..14107 /gene="RRE" /SECDrawAs="Gene" /SECStyleId=1 /X-Transferred_From="Plasmid_p119 (modified)" /X-Transferred_Similarity="100.00%" /Transferred_From="M1399, Plasmid_p119 (modified)" /Transferred_Similarity="100.00%" /label="RRE CDS" ORIGIN 1 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 61 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 121 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 181 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 241 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 301 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 361 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 421 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc 481 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg 541 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa 601 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat 661 ccagtttggt taattaaggg tgcagcggcc tccgcgccgg gttttggcgc ctcccgcggg 721 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga 781 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc 841 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt 901 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg 961 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct 1021 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca 1081 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt 1141 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg 1201 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg 1261 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg 1321 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat 1381 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggg 1441 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc 1501 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca 1561 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt 1621 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga 1681 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag 1741 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca 1801 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg 1861 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga 1921 ctctagagga tccccgggta ccggtcgcca acgcgtgcca ccatggacaa gaagtacagc 1981 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag 2041 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac 2101 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga 2161 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc 2221 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg 2281 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg 2341 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc 2401 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc 2461 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc 2521 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg 2581 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc 2641 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg 2701 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg 2761 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac 2821 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg 2881 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac 2941 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag 3001 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga 3061 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc 3121 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac 3181 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag 3241 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc 3301 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc 3361 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct 3421 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag 3481 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa 3541 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag 3601 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag 3661 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg 3721 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc 3781 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt 3841 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa 3901 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg 3961 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac 4021 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag 4081 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat 4141 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag 4201 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag 4261 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag 4321 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg 4381 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag 4441 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt 4501 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag 4561 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg 4621 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc 4681 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag 4741 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat 4801 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc 4861 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac 4921 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc 4981 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag 5041 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc 5101 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac 5161 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg 5221 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc 5281 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg 5341 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg 5401 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc 5461 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc 5521 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg 5581 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg 5641 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag 5701 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg 5761 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat 5821 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc 5881 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac 5941 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc 6001 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga 6061 ggcgacaagc gtcctgctgc tactaagaaa gctggtcaag ctaagaaaaa gaaagctagc 6121 ggcagcggcg ccggatcccc aaagaagaaa aggaaggttg aagaccccaa gaaaaagagg 6181 aaggtgataa gcgctggaag cggagctact aacttcagcc tgctgaagca ggctggagac 6241 gtggaggaga accctggacc taccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6301 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 6361 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 6421 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 6481 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 6541 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 6601 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 6661 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 6721 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc 6781 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6841 cgcaagcccg gtgcctgata agaattcgat atcaagctta tcgataatca acctctggat 6901 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6961 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 7021 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 7081 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 7141 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 7201 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 7261 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 7321 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 7381 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 7441 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta 7501 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 7561 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga 7621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 7681 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca 7741 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg 7801 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat 7861 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag 7921 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag 7981 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 8041 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 8101 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 8161 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 8221 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 8281 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 8341 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 8401 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 8461 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 8521 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 8581 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 8641 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 8701 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 8761 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 8821 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 8881 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 8941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 9001 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 9061 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 9121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 9181 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 9241 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg 9301 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact 9361 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag 9421 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg 9481 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc 9541 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt 9601 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg 9661 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg 9721 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg 9781 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 9841 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 9901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 9961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 10021 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 10081 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 10141 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 10201 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 10261 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 10321 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 10381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 10441 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 10501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 10561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 10621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 10681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 10741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 10801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 10861 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 10921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 10981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 11041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 11101 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 11161 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 11221 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 11281 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 11341 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 11401 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 11461 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 11521 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 11581 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 11641 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 11701 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 11761 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 11821 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 11881 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 11941 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 12001 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 12061 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 12121 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 12181 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag 12241 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 12301 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 12361 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag 12421 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 12481 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 12541 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 12601 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 12661 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 12721 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 12781 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 12841 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 12901 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 12961 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg 13021 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 13081 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 13141 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 13201 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc 13261 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt 13321 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt 13381 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg 13441 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc 13501 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca 13561 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct 13621 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga 13681 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg 13741 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa 13801 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa 13861 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg 13921 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc 13981 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct 14041 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac 14101 agctcctggg gatttggg //