LOCUS Construct_15 3928 bp DNA circular UNA 29-APR-2014 DEFINITION CMVp-HHRibo-gRNA1-HDVRibo-pA. ACCESSION urn.local...1397729720443.87 VERSION urn.local...1397729720443.87 KEYWORDS . SOURCE Mammalian ORGANISM Mammalian . FEATURES Location/Qualifiers rep_origin 751..1206 /created_by="User" /label="phage f1 origin of replication" promoter 1335..1923 /Original_Bases /modified_by="User" /label="pmCMV" misc_feature 1888..1894 /created_by="User" /label="TATA box" misc_RNA 1930..1972 /created_by="User" /label="5' Self cleaving Ribozyme" misc_feature 1930..1935 /created_by="User" /label="Complementary to the first 6 gRNA bps" gRNA 1973..1992 /created_by="User" /label="a2m_gRNA" misc_feature 1993..2072 /created_by="User" /modified_by="User" /label="gRNA scaffold" misc_RNA 2073..2140 /created_by="User" /modified_by="User" /label="3' Self cleaving Ribozyme" polyA_signal 2158..2607 /Original_Bases /modified_by="User" /label="HSV polyA" gene complement(3687..619) /created_by="User" /label="AmpR" ORIGIN 1 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 61 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 721 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg 781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 961 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 1021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 1081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 1141 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 1201 caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 1261 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg 1321 tacttggagg tacctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 1381 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 1441 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 1501 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 1561 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 1621 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 1681 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 1741 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 1801 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 1861 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttatg aaccgtcaga 1921 tccgagctcc gactactgat gagtccgtga ggacgaaacg agtaagctcg tctagtcgcg 1981 tgtagcgaag cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca 2041 acttgaaaaa gtggcaccga gtcggtgctt ttggccggca tggtcccagc ctcctcgctg 2101 gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac cccgggatgc tagctaagcg 2161 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 2221 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 2281 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc 2341 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa 2401 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg 2461 gctggcactc tgtcgatacc ccaccgagac gccattgggg ccaatacgcc cgcgtttctt 2521 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg 2581 gggcggcagg ccctgccata gcctcagctc gagtatgtcg accgatgccc ttgagagcct 2641 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 2701 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg 2761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 2821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 2881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 2941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 3001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 3061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 3121 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 3181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 3241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 3301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 3361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 3421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 3481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 3541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 3601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 3661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 3721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 3781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 3841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 3901 cctgcaactt tatccgcctc catccagt //